Cut out the gel slice that includes bands greater than 1 kb for each sample and purify the DNA using QIAquick Gel Extraction kit according to manufacturer's protocol. Use two Qiagen columns for ...
The DNA and RNA are fragmented with enzymes and heat, respectively. QIAGEN’s FastSelect reagent, which is incorporated into the kit, eliminates unwanted RNAs (ribosomal and/or globin ...