The GoTaq ® Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR ... Promega's PCR Systems, enzymes and reagents are proven ...

This technique allows scientists to see the results almost immediately while the process is still ongoing, whereas conventional RT–PCR only provides results at the end of the process. Real time RT–PCR ...

Owing to extensive accumulation of primer-dimers when no template control is used during one-step RT-PCR, we introduced a two-step protocol to eliminate this problem. This study further ...

Quantitative reverse transcriptase (RT) PCR has evolved ... A nested PCR approach was chosen as it produced a cleaner endpoint PCR product than a one-step PCR with an increased number of cycles.