Real-time PCR is the favored method for measuring ... reverse transcription and sample preparation. Some master mixes are optimized for fast reactions, whereas others contain proprietary reagents ...

Abstract Research of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) and Fibromyalgia (FM), two acquired chronic illnesses affecting mainly females, has failed to ascertain their frequent ...

We used the ViiA7 real-time fluorescence quantitative PCR system to measure the expression levels of each reference gene. The reaction system consisted of 20 μL, including 10 μL of Hieff qPCR SYBR ...

Real-time PCR was performed using SYBR Green Realtime PCR Master Mix (TOYOBO) on ABI QuantStudio 6 flex Real-time PCR System (Thermo Fisher Scientific). The RT-qPCR Primer sequences for targeting ...

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as internal controls in SYBR ® Green Realtime PCR Master Mix-Plus kit (Toyobo, Osaka, Japan). Each experiment was performed at least three ...

The 2X PCR Master Mix is a made-to-order custom master mix. Once your order is received, your custom master mix will be delivered in 10 business days. The 2X formulation contains customer defined ...