The back-to-back deaths of two beloved bald eagle siblings stunned the thousands of people who tuned into the livestream of their nest in Florida. Now, biologists have shared they both died from bird flu. The bald eaglets, known as E24 and E25, died within 36 hours of each other, McClatchy News previously reported.

UPDATE: The second bald eaglet, E25, died in the early hours of Jan. 28 a little over a day after the death of its sibling, the Southwest Florida Eagle Cam shared. The eagle started having seizures and fell out of the nest, according to moderators. Wildlife officials collected both bodies for testing. The original story is below.