eLife16d
A dual ribosomal system in the zebrafish soma and germline
Shah and colleagues take advantage of the presence of maternal and somatic ribosomes in zebrafish and confirm their differential expression during development. The authors convincingly show that ...
Frontiers19d
Dextransucrase Expression Is Concomitant with that of Replication and Maintenance Functions of the pMN1 Plasmid in Lactobacillus sakei MN1
For the isolation of total RNA, bacterial cultures were grown ... and then purified following the “RNA Cleanup” protocol of the “RNeasy Midi” kit from QIAGEN. In addition, the samples were subjected ...
eLife7d
Genome organization by SATB1 binding to base-unpairing regions (BURs) provides a scaffold for SATB1-regulated gene expression
Mammalian genomes are organized by multi-level folding, yet how this organization contributes to cell type-specific transcription remain unclear. We uncovered that the nuclear protein SATB1 ...
Frontiers16d
Compressive stress drives adhesion-dependent unjamming transitions in breast cancer cell migration
Cellular unjamming is the collective fluidization of cell motion and has been linked to many biological processes, including development, wound repair, and tumor growth. In tumor growth, the ...
jmg.bmj26d
Novel role for non-homologous end joining in the formation of double minutes in methotrexate-resistant colon cancer cells
The BAC clones, PR11-90A9, PR11-27F9, PR11-42H4 and PR11-957J15 (BAC PAC Resources Center, California, USA), were extracted using Genopure Plasmid Midi Kit (Roche) according to the manufacturer's ...